Yellow mealworm (Tenebrio Molitor) enhances intestinal immunity in largemouth bass (Micropterus salmoides) via the NFκB/survivin signaling pathway.

This study aimed to elucidate the mechanisms of yellow mealworm (Tenebrio Molitor, YM) in intestinal immunity and health. Largemouth bass, as an enteritis modeling animal, were fed 3 diets containing YM at 0% (YM0), 24% (YM24) and 48% (YM48). The YM24 group had reduced levels of proinflammatory cytokines, while the YM48 group experienced a negative impact on intestinal health. Next, the Edwardsiella tarda (E. tarda) challenge test consisted of 4 YM diets, 0% (EYM0), 12% (EYM12), 24% (EYM24), and 36% (EYM36). The EYM0 and EYM12 groups exhibited intestinal damage and immunosuppression by the pathogenic bacteria. However, the above adverse phenotypes were attenuated in the EYM24 and EYM36 groups. Mechanistically, the EYM24 and EYM36 groups enhanced intestinal immunity in largemouth bass via activating NFκBp65 and further upregulating survivin expression to inhibit apoptosis. The results identify a protective mechanism of YM as a novel food or feed source by improving intestinal health.

Fabrication and characterization of highly sensitive flexible strain sensor based on biodegradable gelatin nanocomposites and double strain layered structures with crack for gesture recognition.

Flexible sensors have attracted extensive attention in the field of human-computer interaction. However, it is still a challenging task to realize accuracy gesture recognition with flexible sensor, which requires sensor not only have high sensitivity, but also have appropriate strain detection range. Here, a high gauge factor flexible sensor (gauge factor âˆ¼ 1296 under 12-20 % strain) based on crack structure is reported. The sensor is made of a biodegradable and stretchable gelatin composite combined with fabric bases, with good repeatability (6000 cycles) and a fast response (60 ms). Because of the double-layer structure, it has a suitable detection range (20 % strain). The sensor is manufactured by a screen-printing process, and it has been used to make data gloves and has realized 9 gestures recognition with machine learning algorithm (99.6 % accuracy). In general, this study offers a wearable gestures recognition scheme through the proposed sensor.

A Natural Gas Fermentation Bacterial Meal (FeedKind®) as a Functional Alternative Ingredient for Fishmeal in Diet of Largemouth Bass, Micropterus salmoides.

A 10-week growth study was conducted to evaluate the effect of a natural gas fermentation bacterial meal (FeedKind®, FK) as a fishmeal (FM) alternative in largemouth bass (Micropterus salmoides) (48.0 ± 0.03 g). Four isonitrogenous and isoenergetic diets were formulated including one commercial control (C, 42% FM) and three experimental diets with gradient FK of 3% (FK3, 29%FM), 6% (FK6, 26%FM) and 9% (FK9, 23%FM), respectively. FK-fed groups showed significantly higher SR than that of C group. The WGR and SGR of fish fed FK3 and FK6 were significantly higher than those of FK9, but not statistical different from the C group. FK-fed groups showed higher apparent digestibility coefficients of dry matter and nutrients. Further, FK-fed groups increased the ratio of SOD/MDA in the plasma and liver, and the upregulation of intestinal Keap1 and downregulation of HIF1α was found in FK3. Furthermore, FK-fed groups showed higher microbial richness and diversity. Pearson correlation analysis found that antioxidant relevant biomarkers were negatively correlated with the relative abundance of certain potential beneficial bacteria. In conclusion, supplemented up to 3-6% FK replacing FM in a low FM diet of largemouth bass could increase growth, survival rate, antioxidant capacity, and improve gut microbiota.

Proteomic analysis of hepatic fibrosis induced by a high starch diet in largemouth bass (Micropterus salmoides).

Largemouth bass is sensitive to the dietary starch level and excess starch can induce metabolic liver diseases (MLD). Hepatic fibrosis is a typical pathological phenotype of MLD in largemouth bass, but the molecular basis underlying is largely unclear. This study fed fish with a low or high starch diet for 4 weeks. Liver tissues with or without fibrotic symptoms were recognized through histopathological and molecular markers analysis of hepatic fibrosis, following TMT Quantitative proteomics and conducted Parallel Reaction Monitoring (PRM) analyses. 2455 differentially expressed proteins with 1618 up-regulated and 837 down-regulated were identified in this study. In GO terms, up-regulated proteins were correlated with cytoskeleton organization, supramolecular fiber, cytoskeleton protein binding, and actin-binding, while down-regulated proteins were involved in mainly metabolism-related processes, and molecular binding activity. Down-regulated proteins were enriched in 63 KEGG pathways and concentrated in metabolism-related pathways, especially glucose, lipid, and amino acid metabolism. 70 KEGG pathways of up-regulated proteins mainly included immunity and inflammation-related pathways. The expression trends of 11 differentially expressed proteins were consistent with proteome results by PRM analysis. In conclusion, the development of hepatic fibrosis induced by high starch may be related to multi-signaling pathways, metabolism processes, and targets, which provides important data for further study on revealing the molecular mechanism of hepatic fibrosis.

Boosting CdS Photocatalytic Activity for Hydrogen Evolution in Formic Acid Solution by P Doping and MoS2 Photodeposition.

Formic acid is an appealing hydrogen storage material. In order to rapidly produce hydrogen from formic acid under relatively mild conditions, high-efficiency and stable photocatalytic systems are of great significance to prompt hydrogen (H2) evolution from formic acid. In this paper, an efficient and stable photocatalytic system (CdS/P/MoS2) for H2 production from formic acid is successfully constructed by elemental P doping of CdS nanorods combining with in situ photodeposition of MoS2. In this system, P doping reduces the band gap of CdS for enhanced light absorption, as well as promoting the separation of photogenerated charge carriers. More importantly, MoS2 nanoparticles decorated on P-doped CdS nanorods can play as noble-metal-free cocatalysts, which increase the light adsorption, facilitate the charge transfer and effectively accelerate the hydrogen evolution reaction. Consequently, the apparent quantum efficiency (AQE) of the designed CdS/P/MoS2 is up to 6.39% at 420 nm, while the H2 evolution rate is boosted to 68.89 mmol·g-1·h-1, which is 10 times higher than that of pristine CdS. This study could provide an alternative strategy for the development of competitive CdS-based photocatalysts as well as noble-metal-free photocatalytic systems toward efficient hydrogen production.

A stable lanthanum-based metal-organic framework as fluorescent sensor for detecting TNP and Fe3+ with hyper-sensitivity and ultra-selectivity.

A new Lanthanum-based luminescent metal-organic framework, {[La(H2O)4(HL)]·H2O} (1), has been successfully synthesized by employing 3,3',5,5'-azodioxybenzenetetracarboxylic acid (H4L) as a rigid organic linker through the solvothermal reactions. 1 exhibits a two-dimensional (2D) layered structure and a three-dimensional (3D) supramolecular structure is formed by hydrogen bonds between the layers. Stability studies indicate that 1 has good chemical stability and thermostability. Meanwhile, the Ksv values for TNP is 4.61 × 104 M-1 with the LOD of 4.13 × 10-6 M and the Ksv value for Fe3+ is 1.22 × 104 M-1 with the LOD of 1.72 × 10-5 M, respectively, which demonstrated that 1 exhibits high sensitivity and excellent selectivity for the detection of TNP and Fe3+via fluorescence quenching. Significantly, 1 shows high regenerability after five recycling progress for sensing Fe3+. The possible mechanisms associated with the luminescent quenching are discussed in detail through some relevant experiments and tests, as well as the DFT calculations. Based on the above excellent properties of 1, it will have extremely potential to be used as a dual functional sensor for both detecting TNP and Fe3+ in aqueous solution, simultaneously.

p-p orbital interaction via magnesium isovalent doping enhances optoelectronic properties of halide perovskites.

p-p orbital interaction through Mg(ii) isovalent doping in methylammonium lead chloride perovskite significantly enhances the electronic properties while not affecting the optical bandgap. This chemical behaviour shows promising applications to optoelectronic devices.

Modified Graphitic Carbon Nitride Nanosheets for Efficient Photocatalytic Hydrogen Evolution.

Considerable research efforts have been devoted to develop noble-metal-free cocatalysts coupled with semiconductors for highly efficient photocatalytic H2 evolution as part of the challenge toward solar-to-fuel conversion. Herein, a new cocatalyst with excellent activity in the electrocatalytic H2 evolution reaction (HER) that is based on Co sheathed in N-doped graphitic carbon nanosheets (Co@NC) was fabricated by a surfactant-assisted pyrolysis approach and then coupled with g-C3 N4 nanosheets to construct a 2 D-2 D g-C3 N4 /Co@NC composite photocatalyst by a simple grinding method. As a result of advantages in effective electrocatalytic HER activity, suitable electronic band structure, and rapid interfacial charge transfer brought about by the 2 D-2 D spatial configuration, the g-C3 N4 /Co@NC photocatalyst that contained 4 wt % Co@NC presented a high photocatalytic H2 generation rate of 15.67 µmol h-1 under visible-light irradiation (λ≥400 nm), which was 104.5 times higher than that of pristine g-C3 N4 . The optimum g-C3 N4 /Co@NC photocatalyst showed a high apparent quantum efficiency of 10.82 % at λ=400 nm.

Novel 3-D superstructures made up of SnO(2)@C core-shell nanochains for energy storage applications.

New 1-D SnO(2)@C core-shell nanochains built into 3-D superstructures are presented for the first time as anode materials for lithium-ion batteries. These novel SnO(2)@C core-shell nanochains exhibit desirable lithium storage properties.